Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jan 6;160(1):113–123. doi: 10.1083/jcb.200207113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Effect of antibody cross-linking on association of BACE1A-CFP and YFP-swAPP to DRMs. 10 h after adenovirus infection to express BACE1A-CFP or YFP-swAPP, the cells were labeled for 2 h with [³⁵S]methionine and chased for 2 h in the presence of antibody KG77 (anti-FP) or antibody 7523 (anti-BACE1). The cells were subsequently lysed in 20 mM CHAPS/TNE at 4°C. (A) After flotation in an OptiPrep step gradient, BACE1A-CFP and YFP-swAPP were immunoprecipitated with antibody KG77 from the collected fractions. (B) Quantification; antibody-induced patching significantly increased the amount of APP (n = 3) and of BACE1 (n = 4) in the top two fractions (DRM associated). The amount in the top two fractions was correlated to the total amount of protein in all fractions.