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. 2003 Mar 31;160(7):1115–1127. doi: 10.1083/jcb.200212059

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Drp1 mediates p20-induced mitochondrial fission. (A) Recruitment of endogenous Drp1 to mitochondria. HeLa cells were mock infected (top) or infected with Adp20 (bottom) in the presence of zVAD-fmk, and 17 h post-infection, cells were fixed, double stained with anti-Drp1 (green) and anti-TOM20 (red) antibodies, and imaged by confocal immunofluorescence microscopy. Enlargement of the merged overlay revealed that clusters of Drp1 relocate along mitochondrial filaments before the onset of fission. (B) H1299 cells, H1299 cells treated with TG, or H1299 b5-BCL-2 cells were infected with Adp20+zVAD for 18 h and the mitochondrial fraction was isolated and analyzed for the presence of Drp1 by SDS-PAGE and immunoblotting. The blot was reprobed with anti-TOM20 antibody to demonstrate equal protein loading.