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. 2003 Mar 31;160(7):1055–1068. doi: 10.1083/jcb.200211076

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Two phases of dextran entry during NEBD. (A) Oocyte coinjected with 500, 90, and 70 kD dextrans labeled with Alexa 488, Cy5, and TMR, respectively. Arrowheads mark the start of complete permeabilization also visible by DIC. At 2:00 the 500-kD dextran is equilibrated only to 90% due to its slow diffusion. Small dark circle in the cytoplasm is an oil drop from the injection. Fluorescence images are pseudo colored for easier comparison. Selected frames are shown, for complete sequence see Video 2, available at http://www.jcb.org/cgi/content/full/jcb.200211076/DC1. Bar, 25 μm. Time mm:ss, 0:00 = start of 500-kD dextran entry. (B) Quantitation of mean nuclear (dashed lines in A) fluorescence of time series shown in A, normalized from minimum to maximum values. Arrows mark time points shown in A. (C) As in B, plotted semilogarithmically to compare entry rates.