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. 2003 Mar 31;160(7):1055–1068. doi: 10.1083/jcb.200211076

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Permeabilization wave of the NE during phase II of NEBD. Oocyte injected with Alexa 488 500-kD dextran imaged in 3D over time during maturation. See also Video 5, A and B, available at http://www.jcb.org/cgi/content/full/jcb.200211076/DC1. Bars, 10 μm. (A) Selected optical slice from the 4D dataset showing initiation and spreading of the permeabilization. Arrowheads mark the boundary between permeabilized and intact NE. The nucleolus excludes the dextran and becomes visible in the last two frames. (B) Isosurface visualization of the complete 4D dataset. Intact (light gray) and permeabilized (dark gray) areas of the NE were segmented and reconstructed in 3D. (C) Animal-vegetal optical cross section of the 4D dataset shown in A and B. Permeabilization is initiated between the animal pole and equator of the nucleus (arrowheads). (D) Positions of the initial entry site from 10 experiments analyzed as in C. Distance from the animal pole normalized to the height of the nucleus is plotted on a scheme of the nuclear surface. (E) Line profiles of fluorescence intensity along the primary entry site (shown by a dashed rectangle on A) at different time points. cp, cytoplasm; nu, nucleus.