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. 2003 Jul 21;162(2):281–291. doi: 10.1083/jcb.200212141

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — PAK directs MEK1 S298 phosphorylation in vitro. (A) In vitro kinase assays were performed using recombinant MEK1 and recombinant PAK3 as described in Materials and methods. (B) REF52 cells were transfected with HA-tagged wild-type MEK1 or HA-MEK1 S298A, suspended for 1 h and allowed to adhere to FN for 10 min. Anti-HA immunoprecipitates were analyzed and blotted with p-S298MEK1 (top) or MEK1 (bottom).