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Src inhibition alters the location of phospho-MAPK. REF52 cells were suspended in the presence or absence of 50 μM PP2 and plated on FN for 1 h in the continued presence or absence of PP2 before co-staining for p-MAPK (red) and paxillin (green). The arrows indicate peripheral adhesion complexes containing p-MAPK; arrowheads indicate paxillin-containing focal adhesions. Bar, 10 μm.
