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. 2003 Jul 21;162(2):269–279. doi: 10.1083/jcb.200301041

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Trunk, not vagal, neural crest cells are repelled by Slit2- expressing cells in vitro. (a–d) Trunk and vagal neural crest cells (green) were grown apposed to live control HEK cells (a and c) or Slit2-expressing cells (b and d) (blue DAPI label). Only when trunk neural crest cells (b) were grown with Slit2-expressing cells was there a sharp border (white arrowhead) formed between the two populations. (e–h) A similar experiment performed with dead control or Slit2-expressing cells again shows a border between trunk neural crest cells and the Slit cell ghosts (white arrowhead in f), demonstrating that the repellent activity is membrane bound.