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. 2003 Jul 21;162(2):269–279. doi: 10.1083/jcb.200301041

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Movie stills from time-lapse video microscopy. Trunk neural crest cells exposed to Slit2 migrate further and have a longer total path length than those exposed to control medium. Trunk neural crest cells were labeled with Calcein AM (Molecular Probes) and washed before exposure to control or Slit2 CM. Cultures were time lapsed for ∼2.5 h under a confocal microscope. Images represent stills from a movie taken at the indicated times. Two cells (red) in each movie were manually traced to follow their movements. Their final path length is indicated in yellow in the last frame.