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. 2003 Jul 21;162(2):269–279. doi: 10.1083/jcb.200301041

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Slit2 enhances neural crest cell migration. Trunk neural crest cells exposed to Slit2 migrate for longer distances compared with control exposed neural crest cells. Trunk and vagal neural tubes were cultured overnight on fibronectin. After one day, media was changed to one conditioned by control or Slit2-secreting cells 1 h before video microscopy in a confocal microscope for 3 h. (a) The total path length was determined and normalized to a 2.5-h time period and binned in groups of 100-μm distances traveled. (b) Total path length (total distance traveled, including the various turns made by the cells) of neural crest cells was plotted as cumulative percentages of the distance traveled. (c) Net path length (net distance from starting point) of neural crest cells was plotted as cumulative percentages of the distance traveled.