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. 2003 Jul 21;162(2):173–183. doi: 10.1083/jcb.200302085

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — LMP1 induces cytoplasmic accumulation of E2F4/5. (A and B) E2F4 (A) or HA-tagged E2F5 (B) was expressed alone (1) or coexpressed with GFP-tagged LMP1 (2 and 4) or GFP-tagged CRM1 (3) in SVts8 cells. E2F4 was detected by immunostaining with anti-E2F4 antibody and HA-tagged E2F5 was determined by immunostaining with anti-HA antibody. Cells were treated with 2.5 ng/ml of LMB (4). Histograms on the right side of the micrographs indicate the percentage of nuclei that were positive (N+) or negative (N−) for E2F4/5 expression. (A and B) Arrows indicate cells expressing E2F alone (1) or cells expressing both E2F and GFP-tagged LMP1 (2 and 4) or cells expressing both E2F and GFP-tagged CRM1 (3). (C) Activation of the human Rb gene promoter by E2F4 or E2F5 was blocked by coexpression of LMP1 in SVts8 cells. Expression plasmids encoding the indicated proteins were introduced into SVts8 cells along with 0.7 μg of Rb gene promoter–luciferase construct and with 0.2 μg of MMLV-lacZ plasmid. Luciferase activities were normalized by lac-Z activities. For all panels, error bars indicate SD.

Figure 4. — LMP1 induces cytoplasmic accumulation of E2F4/5. (A and B) E2F4 (A) or HA-tagged E2F5 (B) was expressed alone (1) or coexpressed with GFP-tagged LMP1 (2 and 4) or GFP-tagged CRM1 (3) in SVts8 cells. E2F4 was detected by immunostaining with anti-E2F4 antibody and HA-tagged E2F5 was determined by immunostaining with anti-HA antibody. Cells were treated with 2.5 ng/ml of LMB (4). Histograms on the right side of the micrographs indicate the percentage of nuclei that were positive (N+) or negative (N−) for E2F4/5 expression. (A and B) Arrows indicate cells expressing E2F alone (1) or cells expressing both E2F and GFP-tagged LMP1 (2 and 4) or cells expressing both E2F and GFP-tagged CRM1 (3). (C) Activation of the human Rb gene promoter by E2F4 or E2F5 was blocked by coexpression of LMP1 in SVts8 cells. Expression plasmids encoding the indicated proteins were introduced into SVts8 cells along with 0.7 μg of Rb gene promoter–luciferase construct and with 0.2 μg of MMLV-lacZ plasmid. Luciferase activities were normalized by lac-Z activities. For all panels, error bars indicate SD.