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. 2003 Jul 21;162(2):173–183. doi: 10.1083/jcb.200302085

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — LMP1 induces dissociation of E2F4 from pRB family proteins. (A and B) LMP1-inducible cell line (A) and Svts8 cells (B) were treated with (lane 2) or without (lane 1) 0.5 μg/ml of Ponasteron A for 2 d. Next, cell lysates were immunoblotted directly with antibodies shown right (lysate) or after immunoprecipitated with anti-E2F4 antibody (IP). (C) E2F4-WT or E2F4-WT fused to NLS sequence (NLS–E2F4) or E2F4 ΔNES (68, 70A E2F4s1) was coexpressed with or without GFP-tagged LMP1 in SVts8 cells. E2F4 was detected by immunostaining with anti-E2F4 antibody and the histograms indicating the percentage of nuclei that were positive (N+) or negative (N−) for E2F4 expression were shown. Error bars indicate SD. (D and E) LMP1-inducible cell line was treated with (lane 2) or without (lane 1) 0.5 μg/ml of Ponasteron A for 2 d. Next, cell lysates were immunoblotted directly with antibodies shown right (lysate) or after immunoprecipitated with antibodies shown left (IP).

Figure 6. — LMP1 induces dissociation of E2F4 from pRB family proteins. (A and B) LMP1-inducible cell line (A) and Svts8 cells (B) were treated with (lane 2) or without (lane 1) 0.5 μg/ml of Ponasteron A for 2 d. Next, cell lysates were immunoblotted directly with antibodies shown right (lysate) or after immunoprecipitated with anti-E2F4 antibody (IP). (C) E2F4-WT or E2F4-WT fused to NLS sequence (NLS–E2F4) or E2F4 ΔNES (68, 70A E2F4s1) was coexpressed with or without GFP-tagged LMP1 in SVts8 cells. E2F4 was detected by immunostaining with anti-E2F4 antibody and the histograms indicating the percentage of nuclei that were positive (N+) or negative (N−) for E2F4 expression were shown. Error bars indicate SD. (D and E) LMP1-inducible cell line was treated with (lane 2) or without (lane 1) 0.5 μg/ml of Ponasteron A for 2 d. Next, cell lysates were immunoblotted directly with antibodies shown right (lysate) or after immunoprecipitated with antibodies shown left (IP).