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. 2003 Sep 1;162(5):789–794. doi: 10.1083/jcb.200302124

Figure 4.

Figure 4.

Endo180 promotes filopodia formation in response to uPA and promotes rapid uPA–uPAR mediated activation of Cdc42 and Rac. (A) Endo180 transfected MCF-7 cells were stimulated with or without uPA for 5 min, fixed and immunostained with mAb A5/158 and counterstained with Alexa 555-phalloidin. Bar, 10 μm. Quantitative values shown graphically represent mean ± SEM number of filopodia per cell from a total of 50 randomly chosen cells in two independent experiments. (B) Cdc42 and Rac activation time courses in MCF-7 cells transfected with vector alone (green), Endo180 (red) or Endo180(Ala1468/Ala1469) (blue) and stimulated with uPA. Values represent the mean ± SEM percent change from levels of vector alone cells at zero time point (n = 4–6 experiments). Bottom panels show representative immunoblots. (C) Endo180 expression and Cdc42 activation in siRNA treated MDA-MB-231 cells stimulated with uPA or EGF for 5 min ct, control siRNA; E, Endo180 siRNA. Left panel shows representative blot, right panel shows a graphical representation of mean ± SD percent change from levels in control siRNA treated cells (n = 2 experiments).