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. 2003 Sep 1;162(5):789–794. doi: 10.1083/jcb.200302124

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Endo189 expression does not alter ERK1/2 and Akt phosphorylation or cell surface LRP. (A) ERK1/2 and Akt phosphorylation in MCF-7 cells transfected with vector alone, Endo180 or Endo180(Ala¹⁴⁶⁸/Ala¹⁴⁶⁹) and stimulated with uPA. Blots show phosphorylated ERK1/2 and Akt together with total ERK1/2 and Akt as loading controls. (B) FACS^® analysis of LRP cell surface expression levels in MCF-7 cells transfected with vector alone (green), wild-type Endo180 (red) or Endo180(Ala¹⁴⁶⁸/Ala¹⁴⁶⁹) (blue). Profiles in black represent vector transfected cells incubated with secondary antibody alone.