Figure 2.

Bead responses depend on actin polymerization and EGFR kinase. (A) MTLn3:EGFR cells were treated with 0.1% DMSO, 1 μM cytochalasin D, or 1 μM nocodazole for 1 min, or 1 μM PD153035 for 15 min. BSA beads or EGF beads were then added to the cells for 5 min followed by fixation and staining with rhodamine phalloidin. Total positive responses are reported. Data represent the mean ± SEM of three experiments; n = 50 for each experiment. (B) EGF beads were added simultaneously with 5 nM EGF for three min. The cells were stained and fixed for F-actin. Arrow indicates location of bead in fluorescence image. Bar, 20 μm.