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. 2003 Sep 1;162(5):795–808. doi: 10.1083/jcb.200305065

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — MCs in MI and MII. (A–D) FISH with an MC chromatid-specific PAC probe (red; see Materials and methods) on MI (A and B) and MII (C and D) spreads of a disomic mouse. (Ai) An MC bivalent, remote from mouse centromeres, displays split FISH signals (arrowheads), indicating maintenance of cohesion at sister centromeres. (Bi) Two MCs showing split chromatid signals (arrowheads) are located separate from each other, but each are associated with a mouse pericentric DAPI-bright heterochromatin block (black in ii). Bar, 5 μm. (Ci) An MC displaying sister chromatid signals (arrowhead) in an MII spermatocyte. Bar, 10 μm. (Di) An MII spermatocyte with one MC with two chromatid signals (arrowhead) and a second MC that is present as a single sister chromatid (arrow) due to loss of sister chromatid cohesion during MI. All DAPI images (Aii–Dii) are shown in grayscale. (Ei) IF staining for STAG3 (red) combined with MC FISH (green) on a meiotic MI showing a STAG3 signal at the MC (arrowhead). (Eii) STAG3 channel only.