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. 2003 Sep 1;162(5):851–862. doi: 10.1083/jcb.200305012

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Cdc15p colocalizes with the formin Cdc12p in a medial spot. (A) Time-lapse images of a cdc15-GFP cell (KGY3019). The arrowhead indicates the initial localization of a Cdc15p–GFP spot to the medial region with subsequent incorporation of other Cdc15p patches into this structure. (B) GFP–Cdc15p, expressed under control of nmt81 promoter, and GFP–Cdc15p(1–425), under control of the cdc15 ⁺ promoter, were visualized in live wild-type cells. (C) GFP–Cdc12p was expressed under control of the nmt81 promoter and visualized in live cdc15-CFP cells (KGY3352). (D) Cdc15p was produced under control of the nmt81 promoter in cdc12p-GFP cells (KGY3066), and live cells were imaged (left). Cdc12p was produced under control of the nmt81 promoter in cdc15-GFP cells (KGY3019), and live cells were imaged (right).