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. 2003 Sep 15;162(6):1045–1055. doi: 10.1083/jcb.200302175

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — MT dynamics in the IS. (a) Maximal Z-projection of small pinhole size CLSM images of neuron transfected with EB1::YFP (green) was stained with anti-MAP2 antibody (red). EB1 extensively labeled the IS (arrow). (b and c) CAFM images of EB1::YFP expressing neurons. (b) Individual EB1 dots, which decorate the growing tips of MTs are clearly observed in dendrites, cell body, and axon in a low level of EB1::YFP expression. Note that individual EB1 dots are resolved in the IS (b, arrows; Video 8). (c) In a higher level of expression, EB1::YFP extensively labeled the MTs in the IS (c, arrow), whereas it labeled only tips of MTs in the cell body and dendrites (c, arrowheads; Video 9). (d) CAFM image of MTs stained with antitubulin antibody. Note that MTs in dendrites as well as cell bodies are visualized by CAFM. Arrow indicates MTs in the cell body. Bars, 10 μm.