Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Sep 15;162(6):1135–1147. doi: 10.1083/jcb.200207056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Myostatin is expressed in adult myoblasts derived from satellite cells. (A) Agarose gel electrophoresis of myostatin ORF amplified in a combined RT-PCR reaction using total RNA from adult myoblasts (lane 2). Amplicons are not detected in the absence of template (negative control) (lane 3). 1-kb plus DNA ladder is shown in lane 1. (B) Western analysis from proteins isolated from adult myoblasts cultured for 48 h showing both the full-length (52 kD) and LAP (38 kD) peptides of Myostatin. (C) Representative immunofluorescence showing the presence of Myostatin in wild-type adult myoblasts (i) and absence in myostatin-null (Mstn^−/−) myoblasts (iii). DAPI staining of the nuclei is shown in the corresponding fields (ii and iv).