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. 2003 Sep 15;162(6):1099–1110. doi: 10.1083/jcb.200304093

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Anti-TLR1 and -TLR2 mAbs block the IL-6 response of human PBMCs to araLAM and Pam₃CSK₄. Fresh human PBMCs were preincubated for 30 min with anti-TLR1 mAb (GD2.F4) or anti-TLR2 mAb (11G7) before adding the stimulants. After 18 h of stimulation with 1 μg/ml araLAM, 100 ng/ml Pam₃CSK₄ (A), 10 μg/ml zymosan, or 10 ng/ml phenol LPS (B), supernatants were harvested and IL-6 was measured by ELISA.