Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Apr 14;161(1):89–101. doi: 10.1083/jcb.200212053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Ratio increases in [Ca² ⁺ ]_i from head and flagellum regions (measured in discrete regions of interest as indicated). Images acquired at 40 frames per second with 25-ms individual frame exposure time. (A) Typical spontaneous fluctuations in [Ca²⁺]_i observed in resting sperm. Images above graphs are intensity images; images below graphs are ratio images against the frame immediately before the spontaneous increase occurred. (B) Typical response of an individual sperm to addition of speract to a final concentration of 125 nM. Images above graphs are intensity images; images below are ratio images against the frame immediately before speract addition. Note the markedly higher intensity of the head compared with the tail and that the fold increases in head and tail are comparable.

Figure 2. — Ratio increases in [Ca² ⁺ ]_i from head and flagellum regions (measured in discrete regions of interest as indicated). Images acquired at 40 frames per second with 25-ms individual frame exposure time. (A) Typical spontaneous fluctuations in [Ca²⁺]_i observed in resting sperm. Images above graphs are intensity images; images below graphs are ratio images against the frame immediately before the spontaneous increase occurred. (B) Typical response of an individual sperm to addition of speract to a final concentration of 125 nM. Images above graphs are intensity images; images below are ratio images against the frame immediately before speract addition. Note the markedly higher intensity of the head compared with the tail and that the fold increases in head and tail are comparable.