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. 2003 Apr 14;161(1):89–101. doi: 10.1083/jcb.200212053

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Representative examples of the effect of Ni² ⁺ treatment on speract-induced [Ca² ^+] _i increases in individual sperm heads. Blue traces from sperm treated with 300 μM Ni²⁺ before addition of 500 pM speract (indicated by arrow); red trace from untreated sperm. Changes in [Ca²⁺]_i were determined for individual heads by ratioing their fluorescence (F) against their initial fluorescence (F₀). Images acquired at 10 frames per second with 100-ms individual frame exposure time.