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. 2003 Apr 14;161(1):89–101. doi: 10.1083/jcb.200212053

Figure 8.

Figure 8.

Representative example of the effect of niflumic acid treatment on speract-induced [Ca2 + ]i increases in individual sperm. Sperm treated with 100 μM niflumic acid before addition of 1 nM speract (indicated by arrow). Changes in [Ca2+]i were determined for heads and flagella by ratioing their fluorescence (F) against their initial fluorescence (F0).