Figure 8.
Representative example of the effect of niflumic acid treatment on speract-induced [Ca2 + ]i increases in individual sperm. Sperm treated with 100 μM niflumic acid before addition of 1 nM speract (indicated by arrow). Changes in [Ca2+]i were determined for heads and flagella by ratioing their fluorescence (F) against their initial fluorescence (F0).