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. 2003 Apr 14;161(1):89–101. doi: 10.1083/jcb.200212053

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Representative examples of the effect of IBMX treatment on speract-induced [Ca² ⁺ ]_i increases in individual sperm. (A) Effect of IBMX treatment on Ca²⁺ increase in sperm heads. Images acquired at 10 frames per second with 100-ms individual frame exposure time. (B) Effect of IBMX treatment on [Ca²⁺]_i increase in sperm flagella. Images acquired at 40 frames per second with 25-ms individual frame exposure time. In both A and B, blue traces from sperm treated with 100 μM IBMX before addition of 500 pM speract (indicated by arrow); red trace from non-IBMX–treated sperm. Changes in [Ca²⁺]_i were determined for heads and flagella by pseudo-ratioing their fluorescence (F) against their initial fluorescence (F₀).