Table I.
A comparison of the kinetic characteristics of speract-induced and spontaneous fluctuations in [Ca2 + ]i
Speract | Control | |
---|---|---|
t1/2 increase | 40 ± 5 ms | 100 ± 20 ms |
t1/2 decrease | 75 ± 10 ms | 900 ± 125 ms |
Magnitude | 2.25 ± 0.01 | 6.8 ± 1.1 |
The time to reach half-height of the increase to peak, the decrease to baseline, and the overall magnitude of the fold increase in intracellular [Ca2+]i were measured in individual flagella. Data presented from cells treated with 100 nM speract (n = 34) and untreated controls (n = 21), where n represents a single speract-induced or spontaneous fluctuation.