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. 2003 Apr 14;161(1):119–129. doi: 10.1083/jcb.200212174

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — GDNF induces branching of GFRα1-expressing MDCK cells in three-dimensional collagen gel. (A) Ret/GFRα1- and GFRα1-expressing MDCK cells were grown in collagen gel with GDNF (100 ng/ml), and wild-type MDCK cells were grown in collagen gel with HGF (50 ng/ml). BSA (100 ng/ml) was used as a negative control. Bar, 100 μm. (B) GDNF induces branching of GFRα1 and Ret/GFRα1 cells but not wild-type MDCK cells, which only respond to HGF. Persephin (PSPN; 100 ng/ml) does not induce branching of any MDCK cell line tested. From the total number of cysts in the field, the percentage of cysts with long branches was calculated. Only the branches with the length of more than two cyst diameters were counted. (C) Dose dependency of the GDNF-induced branching of GFRα1- and Ret/GFRα1-expressing MDCK cells. GDNF concentrations are marked per ml. Results are reported as fold of branching cysts over the noninduced control. Means ± SEM of five to eight counted fields are shown. The results are representative of five (A and B) and three (C) independent experiments. (B and C) GDNF significantly increases branching in GFRα1- and Ret/GFRα1-expressing MDCK and HGF increases branching of wild-type MDCK (B) compared with the control media (P < 0.001).