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. 2003 Apr 14;161(1):119–129. doi: 10.1083/jcb.200212174

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — GFRα1 does not complex with Met. Binding of ¹²⁵I-GDNF to COS7 cells transfected with gfrα1 and ¹²⁵I-HGF to wild-type COS7 followed by cross-linking with EDC together with sulfo-NHS. Immunoprecipitates with anti-Met antibodies (IP:Met) were analyzed by SDS-PAGE under reducing conditions. In total lysates (TL), different complexes of ¹²⁵I-GDNF (monomers or dimers) and the dimers of GFRα1 are marked with a square bracket. ¹²⁵I-HGF α subunit and proHGF are marked by arrows. ¹²⁵I-HGF–Met complexes are indicated by arrowheads. The results are representative of five independent experiments.