Figure 2.
PGF 2α-mediated muscle growth occurs through the FP receptor. (A) Primary myoblasts were induced to differentiate in the presence of vehicle, 10−6 M fluprostenol, or 10−6 M AL-8810 and immunostained for EMyHC after 48 h. Bar, 60 μm. (B) Cells were treated with the indicated doses of fluprostenol for 48 h and analyzed as in Fig. 1 E. (C) After 24 h in DM, cells were treated with indicated doses of AL-8810 for 24 h and analyzed as in Fig. 1 E. Data are mean ± SEM of three independent experiments. *Significantly different from vehicle, P < 0.05.