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. 2003 Apr 14;161(1):111–118. doi: 10.1083/jcb.200208085

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — PGF _2α does not induce myotube growth or fusion of NFATC2 ⁻ ^/ ⁻ muscle cells. (A) NFATC2^−/− myoblasts were induced to differentiate and treated with vehicle or 10⁻⁶ M PGF_2α for 48 h, followed by immunostaining for EMyHC. Bar, 60 μm. (B) Nuclear number assays were performed as in Fig. 1 E on NFATC2^−/− cultures treated with vehicle or PGF_2α at a range of doses. Data are the mean ± SEM of three independent cell isolates. (C) FP receptor mRNA expression was examined by RT-PCR after 24 h in DM in wild-type and NFATC2^−/− muscle cells. Representative ethidium bromide staining of acrylamide gel is shown with 18S rRNA as an internal control.