Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Apr 14;161(1):79–88. doi: 10.1083/jcb.200210057

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — PLCδ4/ATL-II is expressed in sperm. (A) The molecular weight of PLCδ4 in brain (B) and sperm (S) was compared by Western blot analysis. 30 μg of each homogenate was subjected to SDS-PAGE. Western blot analysis was performed using an antibody against PLCδ4 raised in the laboratory. (B) The molecular weight of sperm PLCδ4 was compared with PLCδ4 and alternative splicing isoforms of PLCδ4. PLCδ4 (δ4) and splicing isoforms of PLCδ4, PLCδ4/ALT-I (δ4-I) and PLCδ4/ALT-II (δ4-II), were overexpressed in COS-7 cells. 40 μg of lysates was subjected to SDS-PAGE, and then Western blotting was performed. The arrowhead denotes that the sperm protein closely resembles in size the product from the PLCδ4/ALT-II splice variant. (C) The products of RT-PCR using testis RNA were compared in size with authentic products using PLCδ4 (491 bp), PLCδ4/ALT-I (δ4-I; 587 bp), PLCδ4/ALT-II (δ4-II; 533 bp), and PLCδ4/ALT-III (δ4-III; 398 bp) plasmids. The arrowhead denotes that the major testis PCR product coincides in size with that of the PLCδ4/ALT-II splice variant.