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. 2003 Apr 14;161(1):197–209. doi: 10.1083/jcb.200208145

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Effects of rDIII on gene expression. (A) Microarray hybridization. Amplified RNA prepared from MCF-7 cells was cultured in the presence (cy3) or absence (cy5) of rDIII and cohybridized to the microarray containing 83 human cDNAs related to cancer cell metastasis (filled histogram). The open histogram represents RNA isolates where the cy3 and cy5 labels were switched. Ratios below 1.0 were inverted and multiplied by −1 to aid in their interpretation. The MMP-2 gene expression signal exceeded a twofold signal intensity change threshold, independent of the dye label orientation. Results are expressed as the mean ± SD from six fluorescence signals. (B) Semi-quantitative RT-PCR. Changes in MMP-2 expression were assessed on the influence of 185 nM rDIII, 2.5 nM Ln-5, or 0.17 nM EGF, with or without AG1478 or LA1. DMSO was added to the controls. As a control for normalization, GAPDH was amplified similarly to MMP-2. Amplified cDNAs of MMP-2 (504 bp) and GAPDH (516 bp) were resolved by agarose gel electrophoresis and visualized by ethidium bromide staining.