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. 2003 Apr 28;161(2):281–294. doi: 10.1083/jcb.200208092

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Hesperadin quickly overrides the mitotic arrest induced by taxol or monastrol. (A, B, and C) HeLa cells were arrested in nocodazole (330 nM), taxol (10 μM), or monastrol (100 μM). Hesperadin (100 nM) or the solvent DMSO was added, and cells were followed by chromosome spreading (A and B) or immunofluorescence microscopy (C). For immunofluorescence microscopy (C), cells were stained with α-tubulin (left), DNA was counterstained with DAPI (right). (D) Percentage of cells arrested in mitosis in the presence of the different drugs is shown as a function of time. Numbers were obtained from the samples shown in A–C. (E) PtK1 cells were treated with monastrol, and mitotic entry was followed by differential interference contrast microscopy. Selected stills of a time-lapse movie are shown, elapsed time in h:min is shown in the lower right. 500 nM Hesperadin was added at time point 1:10 when the typical monoastral spindle had formed, and mitotic exit was followed. All sister chromatids move into the single polar region.