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. 2003 Apr 28;161(2):429–439. doi: 10.1083/jcb.200210135

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Regulation of Rho, Rac, and Cdc42 activity by shear stress. HUVECs were subjected to shear stress for the indicated times. GTP-loading assays for RhoA, Rac1, and Cdc42 were performed as described in Materials and methods. Panels on the left show fold increase of RhoA, Rac1, and Cdc42 activity during stimulation and were calculated as the amount of GTP-bound protein relative to whole cell lysates. Corresponding representative examples of Western blots of GTP-bound proteins and total cell lysates are shown on the right. Values are means ± SD from four to five independent experiments. *, P ≤ 0.05; **, P ≤ 0.01, comparisons with static control, t test.