Table I.
Purification of CCP MT–depolymerizing activity from Xenopus egg extract
| Step | Total activity | Protein | Specific activity | Fold purification |
|---|---|---|---|---|
| U | μg | U/μg | ||
| HSS | 440,000 | 1,320,000 | 0.33 | 1.00 |
| AS supe | 400,000 | 524,000 | 0.76 | 2.30 |
| Phenyl sepharose | 300,000 | 122,000 | 2.45 | 7.40 |
| Heparin | 150,000 | 2,410 | 62.30 | 188.79 |
| MonoS 1 | 72,000 | 595 | 121.00 | 366.67 |
| Superose 6 | 15,000 | 65 | 230.77 | 699.30 |
| MonoQ FT | 10,000 | 28 | 357.14 | 1,082.24 |
| MonoS 2 | 4,500 | 5 | 900.00 | 2,727.27 |
Activity was defined as the maximal dilution that would depolymerize all microtubules in the visual assay within 10 min (see Materials and methods). HSS, high-speed supernatant; AS supe, 40% AS supernatant.