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. 2003 Apr 28;161(2):371–380. doi: 10.1083/jcb.200209057

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Tmod3 is a novel Tmod isoform expressed in endothelial cells. (A) Tmod3 caps actin filament pointed ends with a similar affinity as Tmods 1 and 4. Pointed-end capping activities of human Tmod3 (□, large dash), human Tmod1 (▴, small dash), and chicken Tmod4 (•, solid). The extent of pointed-end capping is plotted as the initial rate of elongation in the presence of Tmod, divided by the initial rate of elongation for the control actin (R/CR), versus increasing Tmod concentration, such that when R/CR = 0, 100% of pointed ends are capped. (B) Cryosection of rat spleen stained with polyclonal anti-Tmod3 antibodies. Bar, 150 μm. (C) Immunoblot of total rat spleen protein with anti-Tmod3 antibody.