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. 2003 Apr 28;161(2):371–380. doi: 10.1083/jcb.200209057

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Tmod3 is expressed in HMEC-1 cells. (A) Immunoblot of cell lysates or purified recombinant proteins with either anti-Tmod3 polyclonal (left) or anti-Tmod1 monoclonal (right) antibodies. Mg²⁺ ghosts, human erythrocyte membranes (Babcock and Fowler, 1994); Tmod1, purified recombinant human Tmod1 protein; Tmod3, purified recombinant human Tmod3 protein. (B) Quantitation of Tmod3 in HMEC-1 cells. ♦, average counts from duplicate standards of purified recombinant human Tmod3; dashed lines indicate average counts from immunoblot bands of 2 × 10⁵ and 5 × 10⁵ HMEC-1 cells. Line fitted to standards by least squares method was used to determine the amount of Tmod in HMEC-1 cells in the linear range of the assay.