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. 2003 Apr 28;161(2):371–380. doi: 10.1083/jcb.200209057

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Tmod3 caps actin filament pointed ends in vivo. (A–C) Permeabilized cell expressing GFP–Tmod3 stained with DNase I or coumarin–phalloidin and DAPI. (A) Rhodamine–DNase I; (B) DAPI + phallacidin; (C) GFP–Tmod3. (D and E) Permeabilized control-infected cell stained with DNase I (D) and coumarin–phalloidin (E). DAPI staining labels nuclei in the same channel as coumarin–phalloidin. Bar, 15 μm. (F) Quantitation of nonnuclear DNase I staining in control and GFP–Tmod3-expressing cells. Values are averages from >20 cells for each cell type, standard deviation is shown by error bars.