Figure 4.

PI3-kinase and Src mediate Rac activation induced by high androgen concentration. (A) Serum-starved NIH3T3 cells were left untreated or treated for the indicated times with either 0.001 or 10 nM R1881. (B) The cells were left untreated or treated for 2 min with 10 nM R1881 in the absence or presence of PP2 or LY294002 (at 5 and 10 mM, respectively). The chemical inhibitors were added 10 min before the hormonal stimulation. Lysate proteins were submitted to a Rac pull-down assay, and the eluted proteins were immunoblotted with the anti-Rac antibody.