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Molecular analysis of AR-RNA expressed in NIH3T3 and LNCaP cells. Total RNA and poly(A)+ RNA was obtained from growing LNCaP or NIH3T3 cells, respectively. cDNA was synthesized and amplified by PRC using specific primers for the NH2 terminus (A), DNA binding (B), and LBD (C) of mouse AR. The PCR products were submitted to agarose gel. PCR products generated without reverse transcriptase from poly(A)+ RNA of NIH3T3 cells were submitted to the same analysis, as a control (ctrl).
