Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 May 12;161(3):625–639. doi: 10.1083/jcb.200303020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — NCAM120 interacts with spectrin through rafts. Double immunostaining of (A) CHO cells and (B) hippocampal neurons from NCAM^−/− mice transfected with NCAM120, NCAM140, or NCAM180 with antibodies against NCAM and spectrin after treatment with MCD. Note that MCD disrupts the association of spectrin with NCAM120, but not with NCAM140 or NCAM180. Bars: (low power) 10 μm; (high power) 5 μm. (C) Immunoblots of lysates from transfected CHO cells reveal comparable NCAM isoform expression. GFP-transfected cells do not express NCAM. (D) Lysates from NCAM-transfected CHO cells were precipitated with NCAM antibodies (NCAM) or rabbit IgG (Ig) and assayed for spectrin by immunoblotting. Note that MCD treatment prevents the precipitation of spectrin with NCAM120, but not with NCAM140 or NCAM180.

Figure 4. — NCAM120 interacts with spectrin through rafts. Double immunostaining of (A) CHO cells and (B) hippocampal neurons from NCAM^−/− mice transfected with NCAM120, NCAM140, or NCAM180 with antibodies against NCAM and spectrin after treatment with MCD. Note that MCD disrupts the association of spectrin with NCAM120, but not with NCAM140 or NCAM180. Bars: (low power) 10 μm; (high power) 5 μm. (C) Immunoblots of lysates from transfected CHO cells reveal comparable NCAM isoform expression. GFP-transfected cells do not express NCAM. (D) Lysates from NCAM-transfected CHO cells were precipitated with NCAM antibodies (NCAM) or rabbit IgG (Ig) and assayed for spectrin by immunoblotting. Note that MCD treatment prevents the precipitation of spectrin with NCAM120, but not with NCAM140 or NCAM180.