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. 2003 Jun 9;161(5):933–944. doi: 10.1083/jcb.200211118

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — ROS are mediators of cell adhesion. (A) 10⁵ cells were serum starved for 24 h before detaching and maintained in suspension for 30 min at 37° with or without 5 μM DPI or 10 μM NDGA. Cells were then kept in suspension or seeded onto fibronectin-treated dishes for 60 min. Photographs of PFA-fixed cells were taken in a phase–contrast microscope. (B) The cells are treated as in A except that they are pretreated with 20 μM NAC (instead of NDGA or DPI) for 30 min in suspension. These experiments are representative of at least three others with similar results.

Figure 4. — ROS are mediators of cell adhesion. (A) 10⁵ cells were serum starved for 24 h before detaching and maintained in suspension for 30 min at 37° with or without 5 μM DPI or 10 μM NDGA. Cells were then kept in suspension or seeded onto fibronectin-treated dishes for 60 min. Photographs of PFA-fixed cells were taken in a phase–contrast microscope. (B) The cells are treated as in A except that they are pretreated with 20 μM NAC (instead of NDGA or DPI) for 30 min in suspension. These experiments are representative of at least three others with similar results.