Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jun 9;161(5):991–1000. doi: 10.1083/jcb.200303192

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Gli localization at epithelial tricellular corners is dependent on Nrx. All embryos are stained for Gli and are at stage 15 of development. (A and B) Wild-type embryos. (C–F) Nrx ⁴⁶ homozygous mutant embryos. (A) Gli localizes to the tricellular corners (arrow) of epithelial cells (en face view). (B) Gli, at tricellular corners, is restricted to the apical half of the lateral membrane domain (cross section view). The apical (a) and basal (b) surfaces of an epithelial cell are marked. (C and D) Severe Nrx ⁴⁶ homozygous mutant embryo. (C) Gli fails to localizes to the tricellular corners of epithelial cells (arrow) and is distributed around the circumference (en face view). In this late stage embryo, dorsal closure has failed (asterisk). (D) Cross section view of the epidermis in C. Rather than being restricted to the apical half of the lateral membrane, Gli extends to the basal surface. (E and F) Nrx ⁴⁶ homozygous mutant embryo with a weak dorsal closure phenotype. (E) En face view of the epidermis. In these mutants, the epidermis appears more wild type, but Gli does not localize to tricellular corners (arrow). (F) Cross section view of the epidermis in E. As in D, Gli is not concentrated to the pSJ domain at tricellular corners, but diffuses basally. Bar, 10 μm.