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. 2003 Jun 23;161(6):1143–1150. doi: 10.1083/jcb.200210069

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — An Eps15 mutant inhibits Tf endocytosis but not FcR-mediated phagocytosis. RAW264.7 cells transiently expressing GFP-Eps15DIIIΔ2 (top) or the dominant-negative construct GFP-Eps15DIII (bottom) were incubated for 30 min at 37°C in medium containing 60 nM Texas red-Tf. Cells were then fixed and analyzed by confocal microscopy. A medial optical section is shown. Left, GFP; right, Texas red-Tf. The asterisks indicate GFP-positive transfected cells. Bar, 10 μm. (B and C) Inhibition of receptor-mediated endocytosis does not block phagocytosis. Association (B) and phagocytic (C) efficiency of cells expressing GFP-Eps15DIIIΔ2 or GFP-Eps15DIII were compared with the efficiency of phagocytosis of nontransfected (NT) control cells, as described in Fig. 1 and Materials and methods. Data presented are the mean ± SEM of three independent experiments.

Figure 2. — An Eps15 mutant inhibits Tf endocytosis but not FcR-mediated phagocytosis. RAW264.7 cells transiently expressing GFP-Eps15DIIIΔ2 (top) or the dominant-negative construct GFP-Eps15DIII (bottom) were incubated for 30 min at 37°C in medium containing 60 nM Texas red-Tf. Cells were then fixed and analyzed by confocal microscopy. A medial optical section is shown. Left, GFP; right, Texas red-Tf. The asterisks indicate GFP-positive transfected cells. Bar, 10 μm. (B and C) Inhibition of receptor-mediated endocytosis does not block phagocytosis. Association (B) and phagocytic (C) efficiency of cells expressing GFP-Eps15DIIIΔ2 or GFP-Eps15DIII were compared with the efficiency of phagocytosis of nontransfected (NT) control cells, as described in Fig. 1 and Materials and methods. Data presented are the mean ± SEM of three independent experiments.