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. 2003 Jun 23;161(6):1093–1103. doi: 10.1083/jcb.200210169

Table I.

Adipose tissue of perilipin -null animal contains HSL of normal specific activity

Animal Wild type perilipin-null
Densitometry
(arbitrary units)		 16,900 13,000
Activity
(nmol/h × mg protein−1)		 686 518
Specific activity 25% 25%

Aliquots of infranatants from adipose tissue of wt and perilipin-null mice were tested for lipolytic activity as described under Materials and methods. Equivalent cellular aliquots were electrophoresed under SDS-PAGE, im-munoblotted with affinity-purified anti-HSL, and scanned with a den-sitometer. The values for densitometric scans were divided by the activity values to give specific activities, revealing that the samples from both types of animals had identical specific activities.