Figure 1.

Kinetics of Rac-1 [³H]GDP/GTP exchange in the presence of anti-Vav immunoprecipitates. Bound Rac-1 [³H]GDP was measured at 10-min intervals by a filter immobilization assay in which samples were filtered through nitrocellulose filters and filter-associated radioactivity was assessed by liquid scintillation counting. ♦, 32Dcl3 cells; ■, BCR/ABL-expressing 32Dcl3 cells. Results are representative of two experiments.