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. 2003 Jun 23;161(6):1067–1079. doi: 10.1083/jcb.200303048

Table I. Distribution of GM130 in daughter cells.

Median deviation from equality

No injection
p97/p47wt
p97/p47(S140A)
Cytokinesis
3.62% 3.98% 3.60%
(n = 70) (n = 67) (n = 68)

NRK cells at prophase (or early prometaphase) were microinjected with either p97/p47wt or p97/p47(S140A) as in Fig. 7 A. The cells were fixed 90 min after injection and stained with anti-GM130 antibodies. Uninjected NRK cells were used as control. Total fluorescence on each side of the equatorial plate in each daughter cell pair at cytokinesis was quantified, and background fluorescence was subtracted. For each pair of values, the percentage of deviation from 50% was calculated, and the median deviation was determined from the number (n) of pairs evaluated.