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. 2002 Jul 8;158(1):165–174. doi: 10.1083/jcb.200112015

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Polysome analysis of VEGF mRNA. (A) The distribution of RNA from MDA-MB-435/β4 and mock transfectants that had been fractionated on sucrose gradients as described in the Materials and methods was determined by measuring the A₂₅₄ of each fraction. (B) The relative VEGF mRNA content of each sucrose gradient fraction was measured by real-time PCR as described in the Materials and methods. Fraction 1 contains unbound RNA present in the ribonucleoprotein fraction, fraction 2 contains 40S and 60S monosomes, fraction 3 contains 80S monosomes, fractions 4–7 contain light polysomes, and fractions 8–12 contain heavy polysomes. The data are presented as the mean ratio of VEGF to β-actin mRNA (± SD) obtained from triplicate samples. Similar results were obtained from three independent experiments.