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. 2002 Jul 8;158(1):153–164. doi: 10.1083/jcb.200201105

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Equibiaxial stretch inhibits lamellipodia formation. Rat VSM cells were plated for 3h on collagen-coated silicone membranes in the equibiaxial stretch device. Cells were then stretched to increase area by 15%. (A) At indicated time points cells were fixed and stained with rhodamine-phalloidin. Cells with representative morphologies are shown. Bar, 20 μm. (B) The portion of the cell perimeter occupied by lamellipodia is expressed as percent of the total perimeter. Values are means ± SEM, for 20 cells per data point from three independent experiments. (C) Time-lapse images of VSM cells were recorded and total cell areas before and at various times after stretch were quantified. Values are means ± SEM for 27 cells.