Figure 7.

Effects of Y-27632 and ML-7 on Rac activity. VSM cells were spread on collagen I–coated coverslip for 16 h, and were incubated with indicated concentrations of Y-27632 (A) or ML-7 (B) for 1 h. Cells were then lysed and GTP-bound Rac and total Rac was detected using the pull-down assay. For densitometric quantification, values are means ± SEM from three experiments. * and ** denote P < 0.02 or <0.05 relative to control, respectively. (C) VSM cells treated with indicated amounts of Y-27632 or ML-7 for 1 h were fixed and stained with rhodamine-phalloidin. Bar, 20 μm.