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. 2002 Jul 8;158(1):91–102. doi: 10.1083/jcb.200201053

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — CPY* degradation is completely blocked upon inhibition of both the HRD/DER pathway and ER-Golgi transport. hrd1Δ (KHY171), hrd1Δ der1Δ (KHY237), erv29Δ (KHY270), erv29Δ hrd1Δ (KHY279), sec12–4 (KHY306), sec12–4 hrd1Δ (KHY308), and wild-type (KHY163) cells were radiolabeled, chased, and CPY* immunoprecipitated at various times. Samples were separated by SDS-PAGE, visualized by fluorography, and quantitated using a Phosphorimager as performed previously (Hill and Cooper, 2000). (A) HRD/DER pathway–deficient strains (hrd1Δ, hrd1Δ der1Δ) and wild-type cells expressing single copy levels of CPY*. (B) hrd1Δ, erv29Δ, hrd1Δ erv29Δ, or wild-type strains expressing single copy levels of CPY*. (C) hrd1Δ, sec12–4, hrd1Δ sec12–4, or wild-type strains expressing single copy levels of CPY* (34°C).