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. 2002 Jul 8;158(1):31–37. doi: 10.1083/jcb.200203022

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — MTs interact with f-actin bundles. (a and b) MTs align along f-actin bundles in the cell body and grow toward dense f-actin plaques. (a) F-actin bundles (highlighted with red lines) radiate out from an f-actin plaque. (b) Dual wavelength images taken from Video 6 (available at http://www.jcb.org/cgi/content/full/jcb.200203022/DC1). MT ends (green, arrowhead and small arrow) grow toward the f-actin plaque while remaining aligned along f-actin bundles (red). As the end of a MT (arrowhead) moves toward the plaque, a speckle along the MT shaft (large arrow) remains stationary with respect to the f-actin bundle. The figure is oriented with the leading edge toward the top of the figure. Elapsed time in min:s. Bar, 10 μm. (c–e) MTs with nondynamic ends move coordinately with f-actin bundles. (c) Time-lapse series from Video 7 (available at http://www.jcb.org/cgi/content/full/jcb.200203022/DC1) of an MT end (green; small arrowhead) moving coordinately with an f-actin bundle (red, highlighted by white line). A nearby nonbundle actin speckle stays stationary over the same time (large arrow). (d) Plot of X and Y coordinates (increasing time from left to right) of the MT end (c, small arrowhead) and two speckles in the f-actin bundle (c, arrows 1 and 2). The MT and the f-actin speckles have the same trajectories and instantaneous velocities. (e) The distance between the MT end (c, small arrowhead) and a speckle on the MT shaft (c, large arrowhead) remains constant. Absent measurements in the series were slightly out-of-focus frames. The figure is oriented with the leading edge toward the top of the figure. Elapsed time in min:s. Bar, 4 μm.